Related Experiment Video
Updated: Jul 29, 2025
08:31
A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
Published on: March 20, 2019
7.6K
DNA Nanopore-Tethered Gold Needle Electrodes for Channel Current Recording.
Shogo Ikarashi1, Hiromu Akai1, Hiroki Koiwa1
1Department of Mechanical Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
ACS Nano
|May 24, 2023
Summary
Researchers developed a new method for inserting synthetic DNA nanopores into lipid membranes, overcoming previous challenges. This advancement facilitates their use in advanced nanopore sensors.
Area of Science:
- Biophysics
- Nanotechnology
- Synthetic Biology
Background:
- Synthetic DNA nanopores offer tunable structures for sensing applications.
- Efficient insertion into planar bilayer lipid membranes (pBLMs) is a significant challenge.
- Existing hydrophobic modifications can cause undesirable DNA aggregation.
Purpose of the Study:
- To develop an efficient method for inserting DNA nanopores into pBLMs.
- To enable reliable channel current measurements of inserted DNA nanopores.
- To advance the application of DNA nanopores in stochastic nanopore sensors.
Main Methods:
- Forming a pBLM on a DNA nanopore-tethered gold electrode tip.
- Utilizing a layered bath solution (oil/lipid mixture and electrolyte).
- Designing and immobilizing a six-helix bundle DNA nanopore structure onto the electrode.
Main Results:
- Achieved efficient physical insertion of electrode-tethered DNA nanopores into pBLMs.
- Successfully measured channel currents of the inserted DNA nanopores.
- Demonstrated a high probability of DNA nanopore insertion using the novel method.
Conclusions:
- The developed method efficiently inserts DNA nanopores into pBLMs.
- This technique overcomes aggregation issues associated with traditional hydrophobic modifications.
- The method is expected to accelerate the development and application of DNA nanopore-based sensors.
