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Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
11:26

Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles

Published on: June 8, 2012

Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human

J F Mornex, Y Martinet, K Yamauchi

    The Journal of Clinical Investigation
    |July 1, 1986
    PubMed

    Insights

    Alveolar macrophages express the c-sis gene, producing platelet-derived growth factor (PDGF). This suggests PDGF plays a role in lung defense and inflammation, particularly during monocyte differentiation into macrophages.

    Area of Science:

    • Cell Biology
    • Immunology
    • Molecular Biology

    Background:

    • Alveolar macrophages are key immune cells in the lungs.
    • Proto-oncogenes like c-sis are involved in cell growth and differentiation.
    • Platelet-derived growth factor (PDGF) has known roles in cell signaling.

    Purpose of the Study:

    • To investigate the expression of the c-sis gene in alveolar macrophages.
    • To determine if alveolar macrophages produce PDGF.
    • To explore the role of c-sis and PDGF in lung defense and inflammation.

    Main Methods:

    • Analysis of mRNA transcripts in alveolar macrophages using complementary DNA.
    • Detection of PDGF-like mediator release from alveolar macrophages.
    • In vitro differentiation of monocytes to study c-sis expression.

    Main Results:

    • Normal and diseased alveolar macrophages spontaneously expressed c-sis mRNA.
    • These cells released a mediator with PDGF properties (chemotactic, mitogenic, receptor binding, antibody precipitation).
    • Blood monocytes lacked c-sis mRNA, but expressed it upon differentiation into macrophages.

    Conclusions:

    • c-sis gene expression and PDGF production occur during monocyte to alveolar macrophage differentiation.
    • PDGF produced by alveolar macrophages may contribute to normal lung defense mechanisms.
    • The c-sis/PDGF pathway is implicated in lung inflammation processes.

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