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Updated: Jul 29, 2025

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays
Published on: April 26, 2024
Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl-CoA synthetase inhibitor
Nicholas D DeBouver1, Madison J Bolejack1, Taiwo E Esan2
1UCB Pharma, 7869 NE Day Road West, Bainbridge Island, WA 98102, USA.
Abstract:
The compound ethyl-adenosyl monophosphate ester (ethyl-AMP) has been shown to effectively inhibit acetyl-CoA synthetase (ACS) enzymes and to facilitate the crystallization of fungal ACS enzymes in various contexts. In this study, the addition of ethyl-AMP to a bacterial ACS from Legionella pneumophila resulted in the determination of a co-crystal structure of this previously elusive structural genomics target. The dual functionality of ethyl-AMP in both inhibiting ACS enzymes and promoting crystallization establishes its significance as a valuable resource for advancing structural investigations of this class of proteins.
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