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Updated: Jul 29, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
Published on: May 30, 2025
CRISPR-dCas9 system for epigenetic editing towards therapeutic applications
Gargi Bhattacharjee1, Nisarg Gohil1, Deepak Siruka1
1Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India.
Abstract:
In the past few decades, epigenetics has emerged as an important area of study to enable a better understanding of gene expression and its regulation. Due to epigenetics, stable phenotypic changes have been possible without alterations in DNA sequences. Epigenetic changes may occur due to DNA methylation, acetylation, phosphorylation and other such mechanisms which alter the level of gene expression without making any difference to DNA sequences. In this chapter, CRISPR-dCas9 used to bring about epigenome modifications for regulating gene expression towards a therapeutic approaches for treating human diseases have been discussed.
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