Highly sensitive and ratiometric detection of nitrite in food based on upconversion-carbon dots nanosensor

Nitrite (NO₂^-) is extensively found in the daily dietary environment. However, consuming too much NO₂^- can pose serious health risks. Thus, we designed a NO₂^--activated ratiometric upconversion luminescence (UCL) nanosensor which could realize NO₂^- detection via the inner filter effect (IFE) between NO₂^--sensitive carbon dots (CDs) and upconversion nanoparticles (UCNPs). Due to the exceptional optical properties of UCNPs and the remarkable selectivity of CDs, the UCL nanosensor exhibited a good response to NO₂^-. By taking advantage of NIR excitation and ratiometric detection signal, the UCL nanosensor could eliminate the autofluorescence thereby increasing the detection accuracy effectively. Additionally, the UCL nanosensor proved successful in detecting NO₂^- quantitatively in actual samples. The UCL nanosensor provides a simple as well as sensitive sensing strategy for NO₂^- detection and analysis, which is anticipated to extend the utilization of upconversion detection in food safety.