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Updated: Jul 29, 2025

A Practical Guide to Phylogenetics for Nonexperts
Published on: February 5, 2014
itol.toolkit accelerates working with iTOL (Interactive Tree of Life) by an automated generation of annotation files
Tong Zhou1,2,3, Kuidong Xu1,2,3, Feng Zhao1,2,3
1Laboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Summary:
iTOL is a powerful and comprehensive phylogenetic tree visualization engine. However, adjusting to new templates can be time-consuming, especially when many templates are available. We developed an R package namely itol.toolkit to help users generate all 23 types of annotation files in iTOL. This R package also provides an all-in-one data structure to store data and themes, accelerating the step from metadata to annotation files of iTOL visualizations through automatic workflows.
Availability And Implementation:
The manual and source code are available at https://github.com/TongZhou2017/itol.toolkit.
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