Related Experiment Video

Updated: Jul 29, 2025

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
11:08

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

7.1K

Lysine Trimethylation in Planktonic and Pellicle Modes of Growth in Acinetobacter baumannii

Nicolas Nalpas1, Takfarinas Kentache1,2, Emmanuelle Dé1

  • 1INSA Rouen Normandie, CNRS, Polymers, Biopolymers, Surfaces Laboratory UMR 6270, University of Rouen Normandie, Rouen F-76000, France.

Journal of Proteome Research
|May 25, 2023
PubMed

Insights

This study identifies 84 K-trimethylated proteins in Acinetobacter baumannii, revealing new insights into bacterial physiology and post-translational modifications (PTMs). These findings enhance our understanding of this important nosocomial pathogen.

Area of Science:

  • Microbiology
  • Proteomics
  • Molecular Biology

Background:

  • Acinetobacter baumannii is a significant nosocomial pathogen, frequently causing ventilator-associated infections.
  • Key biological processes in A. baumannii, including pellicle formation, remain poorly understood.
  • Post-translational modifications (PTMs) are increasingly recognized for their crucial roles in bacterial physiology.

Purpose of the Study:

  • To investigate K-trimethylation in Acinetobacter baumannii ATCC 17978.
  • To compare trimethylation patterns in planktonic and pellicle biofilm modes.
  • To establish a comprehensive proteomic resource for A. baumannii trimethylation.

Main Methods:

  • Proteomic analysis of A. baumannii ATCC 17978.
  • Comparison of sample preparation methods (strong cation exchange, antibody-capture).
  • Evaluation of different database search engines for high-confidence peptide identification.

Main Results:

  • Identification of 84 K-trimethylated proteins in A. baumannii.
  • Enrichment of K-trimethylated proteins in DNA/protein synthesis, transport, and lipid metabolism.
  • Observation of co-occurring acetylation and trimethylation at identical lysine residues, suggesting proteoforms and PTM cross-talk.

Conclusions:

  • This is the first large-scale proteomic study of K-trimethylation in A. baumannii.
  • The identified trimethylated proteins offer new targets for understanding A. baumannii pathogenesis.
  • The study provides a valuable resource for future research on bacterial PTMs and virulence.
Keywords:
Acinetobacter baumanniibacteriabiofilmlysine trimethylationpost-translational modificationsproteomics

More Related Videos

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors10:17

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Published on: October 9, 2016

15.4K
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.1K

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
32
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
51
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.5K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.6K