Predicting the Progress of Tuberculosis by Inflammatory Response-Related Genes Based on Multiple Machine Learning
Shuai Ma1,2, Peifei Peng3, Zhihao Duan1,2
1Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443000, China.
Journal of Immunology Research
|May 25, 2023
Summary
This study identifies key inflammatory genes and hsa-miR-3163 involved in tuberculosis progression. Retinoic acid shows potential for preventing latent tuberculosis infection (LTBI) from advancing to active tuberculosis (ATB).
Area of Science:
- Immunology
- Molecular Biology
- Infectious Diseases
Background:
- Tuberculosis (TB) affects a quarter of the global population, with latent TB infection (LTBI) progression to active TB (ATB) posing a significant challenge.
- Current biomarkers for identifying individuals at risk of ATB progression are limited.
- Advanced molecular tools are needed for accurate TB risk stratification.
Purpose of the Study:
- To identify key genes and molecular mechanisms involved in the progression of LTBI to ATB.
- To develop novel diagnostic tools and therapeutic strategies for TB.
- To explore the role of specific miRNAs and potential drug candidates in TB pathogenesis.
Main Methods:
- Machine learning models (LASSO, RF, SVM-RFE) were employed to identify characteristic genes from TB datasets.
- Gene expression, diagnostic accuracy, and correlations with immune cells/checkpoints were analyzed.
- miRNA-gene networks and potential drug targets were predicted.
Main Results:
- 96 upregulated and 26 downregulated inflammatory genes were identified in ATB compared to LTBI.
- Characteristic genes showed excellent diagnostic performance and correlated significantly with immune cells and checkpoints.
- hsa-miR-3163 was implicated in LTBI to ATB progression, and retinoic acid emerged as a potential therapeutic agent.
Conclusions:
- Key inflammatory genes and hsa-miR-3163 are crucial in LTBI to ATB progression.
- The identified genes offer diagnostic potential and highlight the CD274 immune checkpoint as a therapeutic target.
- Retinoic acid presents a promising therapeutic strategy for preventing LTBI progression and treating ATB.
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