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Related Experiment Video

Updated: Jul 29, 2025

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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CRISPR-based functional genomics screening in human-pluripotent-stem-cell-derived cell types.

Kun Li1,2, Miao Ouyang1,2, Jiangshan Zhan1,2

  • 1Department of Medical Neuroscience, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong Province 518055, China.

Cell Genomics
|May 25, 2023
PubMed
Summary
This summary is machine-generated.

CRISPR screening combined with human pluripotent stem cell (hPSC) differentiation enables functional genomics studies in diverse cell types. This approach advances understanding of gene function and disease mechanisms.

Keywords:
CRISPR technologycell differentiationfunctional genomicsgenetic screenshuman pluripotent stem cells

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Area of Science:

  • Genomics
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Transcriptomic profiling advances gene expression knowledge across human cell types.
  • Understanding gene function in specific cell types remains a key challenge.
  • Human pluripotent stem cells (hPSCs) offer a versatile source for various human cell types.

Purpose of the Study:

  • To review the progress of CRISPR-Cas9 functional genomics screening in hPSC-derived cells.
  • To highlight the integration of CRISPR screening with stem cell differentiation.
  • To identify gene functions, disease mechanisms, and therapeutic targets in specific cell types.

Main Methods:

  • CRISPR-Cas9-based functional genomics screening.
  • Differentiation of human pluripotent stem cells (hPSCs) into various cell types.
  • High-throughput analysis of gene function.

Main Results:

  • CRISPR screening in hPSC-derived cells provides systematic gene function analysis.
  • This integration offers unprecedented opportunities to study cell-type-specific gene roles.
  • Potential for identifying disease mechanisms and therapeutic targets.

Conclusions:

  • The combination of CRISPR screening and hPSC differentiation is a powerful emerging field.
  • This approach facilitates the study of gene function across diverse human cell types.
  • Future directions include addressing current challenges and expanding applications for disease research.