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Related Experiment Video

Updated: Jul 29, 2025

DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Decoding protein methylation function with thermal stability analysis.

Cristina Sayago¹, Jana Sánchez-Wandelmer¹, Fernando García¹

¹Proteomics Unit, Spanish National Cancer Research Centre (CNIO), 28029, Madrid, Spain.

Nature Communications

|May 25, 2023

Summary

Protein methylation, a key epigenetic regulator, is explored using thermal stability analysis. This study reveals Prmt5 and Ezh2 functions in mouse stem cells, uncovering new roles in gene regulation and cell division.

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Published on: April 28, 2022

Area of Science:

Biochemistry
Molecular Biology
Epigenetics

Background:

Protein methylation is crucial for cellular functions beyond epigenetics.
Systems-level analysis of protein methylation lags behind other post-translational modifications.
Thermal stability analysis offers a novel method to assess protein functional status.

Purpose of the Study:

To investigate the utility of thermal stability analysis for studying protein methylation.
To explore the roles of Prmt5 and Ezh2 in mouse embryonic stem cells.
To identify novel substrates and functions associated with protein methylation.

Main Methods:

Utilized thermal stability analysis on mouse embryonic stem cells.
Investigated the regulation of mRNA binding proteins by Prmt5.
Examined the function of Ezh2 in mitotic chromosomes and identified potential substrates.

Main Results:

Thermal stability analysis successfully revealed molecular and functional events linked to protein methylation.
Prmt5 was found to regulate mRNA binding proteins involved in liquid-liquid phase separation and stress granule formation.
A non-canonical function of Ezh2 was identified in mitotic chromosomes, with Mki67 proposed as a substrate.

Conclusions:

Thermal stability analysis is a powerful approach for systematic exploration of protein methylation.
The study provides insights into the roles of Prmt5 and Ezh2 in pluripotency and cellular regulation.
This work establishes a valuable resource for understanding protein methylation in stem cell biology.