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Square-wave voltammetry of human blood serum
Pavlinka Kokoskarova1, Leon Stojanov2, Kosta Najkov2
1Faculty of Medical Sciences, Goce Delcev University, Krste Misirkov 10A, 2000, Stip, Republic of North Macedonia.
Scientific Reports
|May 25, 2023
Summary
Advanced square-wave voltammetry enables electrochemical analysis of human blood serum. This technique identifies uric acid, bilirubin, and albumin in a single experiment without sample pretreatment.
Area of Science:
- Electrochemistry
- Analytical Chemistry
- Biomedical Science
Background:
- Human blood serum analysis is crucial for diagnostics.
- Electrochemical methods offer sensitive detection but face challenges in complex biological matrices.
- Developing direct analysis techniques for serum components is essential.
Purpose of the Study:
- To investigate the electrochemical behavior of human blood serum using square-wave voltammetry.
- To demonstrate the capability of an edge plane pyrolytic graphite electrode for analyzing complex biological samples.
- To simultaneously detect uric acid, bilirubin, and albumin in serum without prior treatment.
Main Methods:
- Square-wave voltammetry (SWV) was employed for electrochemical analysis.
- An edge plane pyrolytic graphite (EPPG) electrode was utilized due to its electrocatalytic properties.
- Human blood serum samples were diluted in a phosphate buffer for analysis.
Main Results:
- SWV successfully characterized electrochemical reactions of uric acid, bilirubin, and albumin in human serum.
- Well-defined, separated, and intense voltammetric signals were obtained for all three species.
- Electrode processes were confirmed as surface-confined, indicating efficient adsorption on the EPPG electrode.
Conclusions:
- The EPPG electrode and SWV provide a powerful platform for direct electrochemical analysis of complex biological samples like serum.
- This method allows for simultaneous detection of key biomolecules, offering a rapid and sensitive diagnostic approach.
- The technique minimizes sample pretreatment and electrode fouling, enhancing its practical applicability.
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