Related Experiment Video

Updated: Jul 29, 2025

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
06:46

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

2.8K

Association between mannose binding lectin gene polymorphisms and clinical severity of COVID-19 in children

Dilek Yilmaz1,2, Mustafa Soyoz3, Aslıhan Sahin4

  • 1Department of Pediatric Infectious Diseases, Health Sciences University Tepecik Training and Research Hospital, Izmir, Turkey.

Molecular Biology Reports
|May 25, 2023
PubMed
Abstract

Insights

Mannose-binding lectin (MBL) gene polymorphisms, specifically in the MBL2 exon-1 region, are linked to the severity of COVID-19 in children. The study found a higher frequency of certain MBL2 genotypes in symptomatic pediatric COVID-19 patients.

Area of Science:

  • Immunology
  • Genetics
  • Infectious Diseases

Background:

  • Mannose-binding lectin (MBL) is a key component of innate immunity, activating the complement system's lectin pathway with MBL-associated serine proteases (MASPs).
  • MBL gene variations are known risk factors for infectious disease susceptibility.
  • The role of MBL and MASP-2 in SARS-CoV-2 infection severity requires further investigation.

Purpose of the Study:

  • To investigate the association between MBL2 gene polymorphisms, serum MBL levels, and serum MASP-2 levels with the clinical course of COVID-19 in pediatric patients.
  • To compare these factors between asymptomatic and symptomatic children with COVID-19.

Main Methods:

  • Genotyping of MBL2 gene single nucleotide polymorphisms (SNPs) in promoter and exon 1 regions using PCR and restriction fragment length polymorphism analysis.
  • Quantification of serum MBL and MASP-2 levels via ELISA.
  • Comparison of genetic and serological data between symptomatic and asymptomatic pediatric COVID-19 cases.

Main Results:

  • No significant differences in MBL2 promoter region polymorphisms (rs11003125, rs7096206) between symptomatic and asymptomatic groups.
  • Codon 52 and 57 genotypes were predominantly wild-type AA in all patients.
  • A significantly higher prevalence of MBL2 exon-1 AB and BB genotypes, and B allele, was observed in symptomatic pediatric COVID-19 patients compared to asymptomatic ones (p < 0.001).
  • Serum MBL and MASP-2 levels did not show significant differences between the groups.

Conclusions:

  • Codon 54 polymorphism in the MBL2 gene exon-1 region is associated with a symptomatic course of COVID-19 in children.
  • MBL2 genotype, not serum MBL or MASP-2 levels, appears to influence COVID-19 symptom severity in the pediatric population studied.
Keywords:
GenMannose-binding lectinPediatricsPolymorphismsSARS-CoV-2

More Related Videos

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

11.9K
Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

3.6K

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.3K
Overview of Carbohydrate Metabolism01:19

Overview of Carbohydrate Metabolism

Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
1.2K
Proteoglycans01:05

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
4.0K
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
13.7K
Human Genetics01:28

Human Genetics

Human genetics provides a profound framework for understanding the interplay between genetic predispositions and human psychology. At the heart of this discipline lies the study of how genes influence physical traits, behaviors, and susceptibility to diseases. Each person carries a unique genetic code that subtly or significantly shapes their psychological and behavioral landscape.
The complex relationship between genetics and psychology is observable through common biological components such...
634
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
306