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Updated: Jul 29, 2025

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Published on: January 31, 2020

Reduced CCR5 expression among Uganda HIV controllers.

Brian Nyiro^1,2, Sharon Bright Amanya^3,2, Alice Bayiyana²

¹New Jersey Medical School, Rutgers University, New Jersey, USA.

|May 25, 2023

Summary

This summary is machine-generated.

Reduced CCR5 expression on CD4+ T cells is key for HIV control in Ugandan controllers. This mechanism helps maintain high CD4+ T cell counts in individuals not on antiretroviral therapy.

Keywords:

CCR5 promoter polymorphisms Elite controllers HIV Non-controllers Viremic controllers

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Area of Science:

Immunology
Virology
Genetics

Background:

HIV controllers spontaneously control viral load without antiretroviral therapy (ART).
Mechanisms of HIV control are diverse and not fully understood, varying among individuals.
Reduced CCR5 expression is one proposed mechanism for HIV control.

Purpose of the Study:

To investigate the role of reduced CCR5 expression in HIV control among Ugandan HIV controllers.
To compare CCR5 expression on CD4+ T cells between HIV controllers and treated non-controllers.

Main Methods:

Ex-vivo characterization of CD4+ T cells from archived peripheral blood mononuclear cells (PBMCs).
Comparison of CCR5 expression levels between HIV controllers and treated HIV non-controllers.
Genotyping for specific CCR5-associated single nucleotide polymorphisms (SNPs).

Main Results:

HIV controllers exhibited significantly reduced CCR5 expression on their CD4+ T cells compared to treated non-controllers.
The percentage of CCR5+ CD4+ T cells was similar between groups, indicating reduced expression per cell.
A subset of controllers carried the rs1799987 SNP (associated with reduced CCR5), while non-controllers had the rs41469351 SNP (associated with increased HIV transmission and mortality).

Conclusions:

Reduced CCR5 density on CD4+ T cells plays a crucial role in HIV control among Ugandan controllers.
This mechanism contributes to maintaining high CD4+ T cell counts in ART-naïve HIV controllers.