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Updated: Jul 29, 2025

Mouse Adipose Tissue Collection and Processing for RNA Analysis
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Mouse Adipose Tissue Collection and Processing for RNA Analysis

Published on: January 31, 2018

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An optimized TRIzol-based method for isolating RNA from adipose tissue.

Hongwei Zhang1,2, Yaoming Liu1, Bingcheng Yu2

  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology & Visual Science, Guangzhou, 510060, China.

Biotechniques
|May 26, 2023
PubMed
Summary
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Isolating high-quality RNA from fatty adipose tissue is challenging. This study presents a simplified TRIzol-based protocol for reliable RNA extraction from lipid-rich samples for research.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Adipose tissue presents challenges for RNA isolation due to high lipid content and low cell density.
  • Existing RNA isolation methods are often complex, requiring multiple kits or reagents, hindering widespread adoption.

Purpose of the Study:

  • To develop and present an optimized, accessible protocol for high-quality RNA isolation from recalcitrant adipose tissue.
  • To provide a simplified method using readily available TRIzol reagent for researchers.

Main Methods:

  • The protocol utilizes TRIzol reagent, a common laboratory reagent for nucleic acid isolation.
  • A step-by-step procedure is detailed for processing adipose tissue samples.

Main Results:

Keywords:
Adipose tissueRNA integrityRNA isolationTRIzol

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  • The optimized protocol successfully yields sufficient and qualified RNA from lipid-rich adipose tissue specimens.
  • The extracted RNA is suitable for various downstream molecular biology applications.

Conclusions:

  • This TRIzol-based protocol offers an effective and simplified solution for RNA isolation from challenging adipose tissue samples.
  • The method enhances the accessibility of RNA extraction from adipose tissue for scientific research.