Physiological and Biochemical Properties of Cotton Seedlings in Response to Cu²⁺ Stress

Copper(II) (Cu²⁺) is essential for plant growth and development. However, high concentrations are extremely toxic to plants. We investigated the tolerance mechanism of cotton under Cu²⁺ stress in a hybrid cotton variety (Zhongmian 63) and two parent lines with different Cu²⁺ concentrations (0, 0.2, 50, and 100 μM). The stem height, root length, and leaf area of cotton seedlings had decreased growth rates in response to increasing Cu²⁺ concentrations. Increasing Cu²⁺ concentration promoted Cu²⁺ accumulation in all three cotton genotypes' roots, stems, and leaves. However, compared with the parent lines, the roots of Zhongmian 63 were richer in Cu²⁺ and had the least amount of Cu²⁺ transported to the shoots. Moreover, excess Cu²⁺ also induced changes in cellular redox homeostasis, causing accumulation of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA). Conversely, antioxidant enzyme activity increased, while photosynthetic pigment content decreased. Our findings indicated that the hybrid cotton variety fared well under Cu²⁺ stress. This creates a theoretical foundation for the further analysis of the molecular mechanism of cotton resistance to copper and suggests the potential of the large-scale planting of Zhongmian 63 in copper-contaminated soils.