Fiber Optic Localized Surface Plasmon Resonance Sensor Based on Carboxymethylated Dextran Modified Gold Nanoparticles...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Analytical Chemistry

Background:

• High mobility group box 1 (HMGB1) is a critical biomarker for chronic inflammation, necessitating sensitive and rapid detection methods.
• Current diagnostic tools for HMGB1 often lack the required speed, sensitivity, or reliability for timely clinical intervention.

Purpose of the Study:

• To develop a facile and highly sensitive fiber optic localized surface plasmon resonance (FOLSPR) biosensor for detecting HMGB1.
• To validate the sensor's performance in terms of linear range, response time, detection limit, and accuracy compared to established methods.

Main Methods:

• Modification of gold nanoparticle surfaces with carboxymethyl dextran (CM-dextran) as a bridging molecule.
• Integration of the modified nanoparticles with a fiber optic localized surface plasmon resonance (FOLSPR) biosensor platform.
• Optimization of sensor conditions for HMGB1 detection and kinetic binding analysis.

Main Results:

• The FOLSPR sensor demonstrated a wide linear detection range for HMGB1 from 10^-10 to 10^-6 g/mL.
• Achieved a low detection limit of 43.4 pg/mL (1.7 pM) with a rapid response time of less than 10 minutes.
• Exhibited high correlation coefficients (>0.9928) and validated kinetic binding events comparable to surface plasmon resonance systems.

Conclusions:

• The developed CM-dextran-functionalized FOLSPR biosensor provides a facile, rapid, and sensitive method for HMGB1 detection.
• This biosensor shows significant potential for direct biomarker detection in clinical applications, offering accurate quantification and reliable validation.
• The findings offer new insights into advanced biosensing technologies for inflammatory disease diagnostics.