Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Bioremediation of Uranium

Microbial Bioremediation of Uranium

Microorganisms play a critical role in the transformation and immobilization of uranium in contaminated environments through four main pathways: bioreduction, biosorption, bioaccumulation, and biomineralization. These mechanisms reduce uranium’s toxicity and prevent its migration through groundwater systems, offering sustainable approaches for in situ bioremediation.Bioreduction of UraniumBioreduction is driven by anaerobic bacteria such as certain strains of Geobacter and Shewanella, which use...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Speciation of cesium cation in natural seawater.

Dalton transactions (Cambridge, England : 2003)·2026

Same author

Radiation-response in <i>Deinococcus</i> bacteria: characterization of the transient IrrE-DdrO heterodimer complex.

FEMS microbes·2026

Same author

Quantitative mapping of methionine sensitivity to oxidation in the copper-bound PcuC chaperone.

Redox biology·2026

Same author

Exploring Americium bioaccumulation in Ascophyllum nodosum seaweed through Europium as a surrogate: A kinetic and speciation study.

Journal of inorganic biochemistry·2025

Same author

Contamination of Bivalve <i>Mytilus galloprovincialis</i>, the Case of Radiocobalt in a Context of Environmental Release.

Environmental science & technology·2025

Same author

Knee kinematics during gait in patients with discoid lateral meniscus: a systematic review.

Minerva pediatrics·2025

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

Development of an Efficient FRET-Based Ratiometric Uranium Biosensor.

Sandrine Sauge-Merle¹, Morgane Recuerda¹, Maria Rosa Beccia²

¹Aix Marseille Université, CEA, CNRS, BIAM, UMR7265, IPM, 13108 Saint Paul-Lez-Durance, France.

|May 26, 2023

Summary

Researchers developed a novel genetically encoded biosensor to detect toxic uranium in the environment. This FRET-based tool offers a sensitive and selective method for monitoring uranium levels, crucial for environmental and human health protection.

Keywords:

FRET calmodulin genetically encoded biosensor metal-binding protein engineering selectivity sensing sensitivity uranium

More Related Videos

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Related Experiment Videos

Last Updated: Jul 4, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Area of Science:

Environmental Science
Biotechnology
Analytical Chemistry

Background:

Uranium dispersion in the environment poses health risks.
Efficient methods for measuring bioavailable uranium are lacking.
Monitoring toxic uranium fractions is essential for environmental safety.

Purpose of the Study:

To develop a genetically encoded, FRET-based ratiometric biosensor for uranium detection.
To create a tool for measuring bioavailable uranium in environmental samples.
To address the need for efficient uranium measurement methods.

Main Methods:

Constructed a biosensor by linking fluorescent proteins to calmodulin.
Modified metal-binding sites and fluorescent proteins.
Characterized multiple biosensor versions in vitro.

Main Results:

Developed a uranium biosensor with high affinity and selectivity over other ions (Ca2+, Na+, Mg2+, Cl-).
Achieved a detection limit below WHO drinking water standards for uranium.
Demonstrated robustness to environmental conditions and a good dynamic range.

Conclusions:

The genetically encoded biosensor is a promising tool for whole-cell uranium detection.
Enables monitoring of bioavailable uranium, even in calcium-rich waters.
Facilitates environmental health monitoring and risk assessment.