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Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella.

Mateusz Koselski1, Sebastian N W Hoernstein2, Piotr Wasko1

  • 1Department of Plant Physiology and Biophysics, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, Lublin 20-033, Poland.

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|May 26, 2023
PubMed
Summary
This summary is machine-generated.

Reactive oxygen species (ROS) trigger electrical and calcium signals in plants, crucial for long-distance information transfer. This study reveals how hydrogen peroxide (H2O2) initiates these signals in moss, impacting gene expression and stress responses.

Keywords:
Calcium imagingCell excitabilityCell-to-cell signalingGlutamate receptorHydrogen peroxideStress-related genes

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Area of Science:

  • Plant signaling
  • Cellular communication
  • Biophysics

Background:

  • Electrical and calcium signals are vital for long-distance information transmission in plants.
  • Reactive oxygen species (ROS) waves, alongside electrical and calcium signals, participate in cell-to-cell communication during stress.
  • Limited knowledge exists on ROS-induced systemic electrical/calcium signals in Physcomitrella and their interrelations.

Purpose of the Study:

  • To investigate the ability of ROS to evoke systemic electrical and calcium signals in the model moss Physcomitrella.
  • To explore the relationship between ROS, electrical signals, and calcium signals in this plant model.
  • To understand the role of these signals in transmitting information about ROS presence.

Main Methods:

  • External application of hydrogen peroxide (H2O2) to induce signals.
  • Inhibition studies using lanthanum (calcium channel inhibitor) and EDTA (calcium chelator).
  • Gene expression analysis of stress-related genes.
  • Calcium imaging using the GCaMP3 biosensor in protonema cells.

Main Results:

  • Hydrogen peroxide (H2O2) application induced long-distance electrical signals (membrane potential changes) in Physcomitrella.
  • Signal generation was calcium-dependent, inhibited by lanthanum and EDTA.
  • Electrical signals showed partial dependence on glutamate receptor (GLR) ion channels.
  • Calcium signals propagated slowly (>5 µm/s) in protonema cells with decrement.
  • Upregulation of a stress-related gene was observed in distant tissues 8 minutes post-H2O2 treatment.

Conclusions:

  • External H2O2 can elicit systemic electrical and calcium signals in Physcomitrella.
  • These signals are calcium-dependent and partially involve GLR channels.
  • ROS-induced signaling contributes to long-distance information transfer regarding stress within the plant.