Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel High-Efficacy Antimicrobial Peptides Derived from Myxinidin and their Therapeutic Efficacy in Bacterial Pneumonia.

Journal of medicinal chemistry·2026
Same author

Droplet Microfluidics-Enabled Mitochondrial Transfer from Young to Senescent MSCs to Ameliorate Cellular Senescence.

ACS applied materials & interfaces·2026
Same author

High prevalence and cross-species transmission potential of Entamoeba gingivalis and Trichomonas tenax in humans, dogs, and cats in Guangxi, China.

Acta tropica·2026
Same author

Reagent-Free Molecular Pendulum Biosensor with Antibody-Aptamer Dual Recognition for Protein Analysis.

ACS sensors·2026
Same author

Breaking the hemorrhage-ferroptosis-fibrosis axis: A ROS-responsive iron-trapping hydrogel remodels the local immune microenvironment for IUA therapy.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

AuPt alloy nanowires <i>via</i> heterogeneous doping for enhanced ethanol electrooxidation.

Chemical communications (Cambridge, England)·2026

Related Experiment Video

Updated: Jul 29, 2025

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

6.6K

Cellular-scale proximity labeling for recording cell spatial organization in mouse tissues.

Xu Zhang1,2, Qi Tang1,3, Jiayu Sun1,3

  • 1College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

Science Advances
|May 26, 2023
PubMed
Summary

Quinone methide-assisted identification of cell spatial organization (QMID) expands proximity labeling. This method maps cell interactions across micrometers, revealing spatial organization in tissues.

More Related Videos

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

8.4K
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

13.2K

Related Experiment Videos

Last Updated: Jul 29, 2025

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

6.6K
Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

8.4K
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
17:08

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

13.2K

Area of Science:

  • Cell biology
  • Chemical biology
  • Genomics

Background:

  • Proximity labeling is crucial for studying cell-cell interactions.
  • Current methods have limited labeling radii, hindering analysis of indirect communication and tissue spatial organization.

Purpose of the Study:

  • To develop a novel chemical strategy, QMID, for cell spatial organization analysis with an extended labeling radius.
  • To overcome limitations of existing proximity labeling techniques for studying indirect cell communication and tissue architecture.

Main Methods:

  • Developed QMID, a chemical strategy utilizing quinone methide (QM) electrophiles produced by surface-installed enzymes.
  • QMID enables labeling of proximal cells independent of direct cell-cell contact, with a labeling radius matching cell dimensions.
  • Applied QMID in cell coculture and in vivo mouse spleen models, followed by single-cell RNA sequencing.

Main Results:

  • QMID successfully revealed gene expression changes in macrophages spatially proximal to tumor cells in coculture.
  • Enabled isolation and analysis of proximal CD4+ and CD8+ T cells in mouse spleen.
  • Uncovered distinct cell populations and gene expression patterns within immune niches of specific T cell subtypes.

Conclusions:

  • QMID offers a powerful tool for dissecting cell spatial organization in complex biological systems.
  • The extended labeling radius of QMID facilitates the study of indirect cell communications and tissue microenvironments.
  • QMID provides novel insights into immune cell interactions and organization within specific niches.