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Updated: Jul 29, 2025
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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
Published on: December 15, 2012
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Global analysis of the yeast knockout phenome
Gina Turco1, Christie Chang2, Rebecca Y Wang1
1Calico Life Sciences LLC, South San Francisco, CA, USA.
Science Advances
|May 26, 2023
Summary
We created Yeast Phenome, a comprehensive database of ~14,500 yeast knockout screens. This resource reveals new gene functions and suggests genome organization optimizes gene function.
Area of Science:
- Genomics
- Systems Biology
- Yeast Genetics
Background:
- Budding yeast Saccharomyces cerevisiae has extensive genome-wide phenotypic data.
- Lack of a central repository and consistent metadata hinders integrative analysis of this data.
Purpose of the Study:
- To aggregate and harmonize ~14,500 yeast knockout screens into a central dataset called Yeast Phenome.
- To analyze this dataset to characterize unknown genes and uncover novel biological insights.
Main Methods:
- Data aggregation and harmonization of ~14,500 yeast knockout screens.
- Bioinformatic analysis of the integrated Yeast Phenome dataset.
Main Results:
- Characterization of two previously unknown genes (YHR045W and YGL117W).
- Identification of tryptophan starvation as a common byproduct of chemical treatments.
- Discovery of an exponential relationship between phenotypic similarity and intergenic distance.
Conclusions:
- Yeast Phenome provides a unified resource for yeast genetics research.
- Gene position significantly correlates with function, suggesting evolutionary optimization in both yeast and human genomes.
