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Related Experiment Videos

R-plasmid transfer in vivo: a prospective study.

D J Platt, J S Chesham, K G Kristinsson

    Journal of Medical Microbiology
    |June 1, 1986
    PubMed
    Summary

    This study analyzed coliform bacteria from patient samples, revealing differences in plasmid distribution among Escherichia coli and other enteric bacteria. Researchers developed methods to assess in-vivo R-plasmid transfer potential and rate within the human gut.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Coliform bacteria are common inhabitants of the human gut.
    • Plasmids, particularly R-plasmids, can carry antibiotic resistance genes and transfer between bacteria.
    • Understanding plasmid dynamics in vivo is crucial for managing bacterial infections and antibiotic resistance.

    Purpose of the Study:

    • To investigate plasmid distribution and R-plasmid transfer in coliform bacteria isolated from human patients.
    • To differentiate Escherichia coli from other enteric genera based on plasmid profiles.
    • To propose criteria for calculating in-vivo R-plasmid transfer potential and rate.

    Main Methods:

    • Isolation and characterization of 287 coliform bacteria from 116 rectal swabs/faecal specimens of 113 patients.
    • Plasmid analysis to determine plasmid content and distribution.
    • Resistance transfer (R-transfer) experiments to assess plasmid mobility.

    Main Results:

    • Escherichia coli exhibited distinct plasmid distribution patterns compared to other enteric genera.
    • Criteria were established to quantify in-vivo R-plasmid transfer potential and rate.
    • In 22 patients, multiple coliforms were isolated, with at least one harboring self-transmissible R-plasmids; in-vivo transfer was observed in 2 out of 43 potential instances.

    Conclusions:

    • Plasmid ecology in the human host is complex, with variations observed between different enteric genera.
    • The proposed methods offer a framework for evaluating the in-vivo R-plasmid transfer capacity of bacteria.
    • Evidence suggests that R-plasmid transfer occurs in vivo, contributing to the dissemination of genetic elements within the human microbiome.

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