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Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
Published on: October 31, 2012
Generation of control human iPSC line INSTEMi001-A from PBMCs of a healthy Indian donor
Niharika Patlolla1, Pretty Ponnachan2, Colin Jamora2
1Institute for Stem Cell Science and Regenerative Medicine, (inStem), Bangalore, India; Current affiliation: Eyestem Research, Centre for Cellular and Molecular Platforms (C-CAMP), Bengaluru, India.
Abstract:
Human pluripotent stem cells serve as a robust model system to study disease pathogenesis in a dish and search for various targeted therapeutics. Collection of control lines from healthy individuals are essential for any study. Therefore, we have generated hiPSC line from a healthy male donor after episomal reprogramming of PBMCs. The generated line is pluripotent, had normal karyotype and has a potential of tri-lineage differentiation. The generated line would serve as control line of Asian origin from Indian population.
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