Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

2.9K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
2.9K
Inflammatory Response01:28

Inflammatory Response

2.2K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
2.2K
Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

2.7K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
2.7K
Chronic Pancreatitis I: Introduction01:24

Chronic Pancreatitis I: Introduction

117
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
117
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

11.5K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
11.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Dose Administration Schedule and Orthovoltage X-ray Beam Quality on Survival and Dose Response Relationships in C57BL/6J Mouse Models of Acute Radiation Syndrome.

Radiation research·2026
Same author

Delayed effects of radiation exposure in a C57L/J mouse model of partial body irradiation with ~2.5% bone marrow shielding.

Frontiers in public health·2024
Same author

Survival and Hematologic Benefits of Romiplostim After Acute Radiation Exposure Supported FDA Approval Under the Animal Rule.

International journal of radiation oncology, biology, physics·2023
Same author

Epithelial Responses in Radiation-Induced Lung Injury (RILI) Allow Chronic Inflammation and Fibrogenesis.

Radiation research·2023
Same author

The progression of radiation injury in a Wistar rat model of partial body irradiation with ∼5% bone marrow shielding.

International journal of radiation biology·2023
Same author

Breaking the limit: Biological countermeasures for space radiation exposure to enable long-duration spaceflight.

Life sciences in space research·2022

Related Experiment Video

Updated: Jul 29, 2025

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.3K

Epithelial Responses in Radiation-Induced Lung Injury (RILI) Allow Chronic Inflammation and Fibrogenesis.

Tyler A Beach1, Jacob N Finkelstein2, Polly Y Chang1

  • 1SRI Biosciences, SRI International, Menlo Park, Calfornia 94025-3493.

Radiation Research
|May 27, 2023
PubMed
Summary

Radiation-induced lung injury (RILI) leads to progressive fibrosis. This study reveals that damage to lung epithelium and reduced immune/fibroblast regulation by epithelial cells contribute to this irreversible injury progression.

More Related Videos

Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis
07:08

Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis

Published on: August 31, 2022

1.7K
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
14:48

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

5.2K

Related Experiment Videos

Last Updated: Jul 29, 2025

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.3K
Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis
07:08

Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis

Published on: August 31, 2022

1.7K
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
14:48

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

5.2K

Area of Science:

  • Pulmonary Medicine
  • Radiation Oncology
  • Cell Biology

Background:

  • Radiation-induced lung injury (RILI) is a progressive condition often leading to lethal pulmonary fibrosis.
  • Resident pulmonary epithelial cells play a critical role in lung homeostasis and injury response.
  • The specific contribution of lung epithelium to RILI progression remains incompletely understood.

Purpose of the Study:

  • To investigate the in vivo response of lung epithelium during the progression of radiation-induced pulmonary fibrosis (RIPF) using an unbiased RNA sequencing approach.
  • To identify molecular changes within lung epithelial cells following irradiation that contribute to RIPF.

Main Methods:

  • Whole thorax lung irradiation (WTLI) was administered to C57BL/6J mice.
  • CD326+ lung epithelium was isolated from irradiated and non-irradiated mice at various time points post-irradiation.
  • RNA sequencing, quantitative PCR (qPCR), and immunohistochemistry were used for analysis.

Main Results:

  • Irradiated lung epithelium showed significantly reduced transcripts regulating immune responses and fibroblast activation at 4 weeks post-irradiation.
  • A decrease in alveolar type-2 epithelial cells (AEC2), indicated by diminished pro-surfactant protein C (pro-SPC) expression, was observed.
  • Reduced expression of Cd200 and cyclooxygenase 2 (COX2) in epithelial cells was associated with increased macrophage and fibroblast activation.

Conclusions:

  • Loss of lung epithelial cells and their regulatory functions (e.g., Cd200, COX2) are key drivers in the progression of radiation-induced pulmonary fibrosis.
  • Strategies aimed at preventing epithelial cell loss or restoring epithelial-derived mediators could be therapeutic for RILI.