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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Updated: Jul 29, 2025

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

Technologies for Vitrification Based Cryopreservation.

Mohammad Amini¹, James D Benson¹

¹Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada.

Bioengineering (Basel, Switzerland)

|May 27, 2023

Summary

This summary is machine-generated.

Cryopreservation, especially vitrification, is vital for preserving biological materials. New research explores heat and mass transfer to improve cryopreservation success and ensure biospecimen supply chain sustainability.

Keywords:

bioengineering cryobiology heat transfer physical chemistry technology vitrification

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Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

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Published on: November 18, 2011

Area of Science:

Biotechnology
Materials Science
Biomedical Engineering

Background:

Cryopreservation enables long-term storage of biological materials like cells and tissues, crucial for modern medicine.
Vitrification is a popular cryopreservation method due to its cost-effectiveness and speed, but faces challenges like intracellular ice formation.
Advancements in cryopreservation are driven by the need to enhance sample viability and functionality post-storage.

Purpose of the Study:

To review the physiochemical aspects of freezing in cryopreservation.
To catalog and present classical and novel cryopreservation approaches.
To highlight the role of interdisciplinary studies in advancing cryopreservation.

Main Methods:

Review of existing literature on cryopreservation principles and techniques.
Analysis of physiochemical factors influencing freezing and vitrification.
Cataloging of traditional and emerging cryopreservation methods and devices.

Main Results:

Cryopreservation techniques, particularly vitrification, are essential for various medical applications.
Intracellular ice formation remains a key challenge in conventional cryopreservation.
Emerging technologies leverage physical and thermodynamic principles for improved cryopreservation outcomes.

Conclusions:

Interdisciplinary research is key to overcoming cryopreservation challenges.
Optimizing heat and mass transfer is critical for successful cryopreservation.
Advancements in cryopreservation contribute to a sustainable biospecimen supply chain.