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Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications.

Andreea Biehl1,2, Ramair Colmon1,2, Anastasia Timofeeva3

  • 1Joint Department of Biomedical Engineering, North Carolina State University & University of North Carolina-Chapel Hill, 4130 Engineering Building III, Campus Box 7115, Raleigh, NC 27695, USA.

Bioengineering (Basel, Switzerland)
|May 27, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a scalable platform to mimic vocal fold mechanical environments in vitro. This tool aids in understanding vocal fold tissue responses to mechanical stimuli for improved treatment strategies.

Keywords:
bioreactordisplacementfibroblastsfrequencygene expressionmesenchymal stem cellspiezoelectric speakervibrationvocal fold

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • Vocal folds (VFs) undergo constant mechanical stress, altering their biomechanical properties.
  • Understanding VF cellular responses in a controlled mechanical setting is crucial for developing effective long-term treatments.

Purpose of the Study:

  • To design, develop, and characterize a scalable, high-throughput platform simulating the in vitro mechanical microenvironment of vocal folds.
  • To enable the study of cellular responses to phonatory stimuli in a controlled manner.

Main Methods:

  • A novel platform was created using a 24-well plate with a flexible membrane over a piezoelectric speaker waveguide.
  • Laser Doppler Vibrometry (LDV) characterized membrane displacements under various vibratory regimes.
  • Human vocal fold fibroblasts and mesenchymal stem cells were cultured and exposed to different mechanical stimuli.

Main Results:

  • The platform successfully exposed cells to tunable vibratory frequencies mimicking phonatory stimuli.
  • Gene expression analysis revealed cellular responses, including pro-fibrotic and pro-inflammatory markers.
  • The platform's design supports scalability, accommodating standard 6- to 96-well plate formats, surpassing current bioreactor limitations.

Conclusions:

  • The developed platform offers a scalable and modular solution for in vitro mechanical stimulation of vocal fold cells.
  • This technology facilitates the characterization of cellular behavior under physiologically relevant mechanical conditions.
  • It provides a valuable tool for advancing research in vocal fold repair and regenerative medicine.