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Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Refined Techniques for Enabling Long-Term Cryo-Repository Using Vitrification and Laser Warming.

Chiahsin Lin^1,2, Wen-Chung Hsieh³, Kanokpron Loeslakwiboon²

¹National Museum of Marine Biology & Aquarium, Pingtung 94450, Taiwan.

Bioengineering (Basel, Switzerland)

|May 27, 2023

Summary

This study introduces a novel cryojig for improved vitrification and laser warming of cells. The refined device achieved 95% laser accuracy and 62% rewarming success, enhancing cryopreservation outcomes.

Keywords:

cryo-jig cryobanking cryopreservation

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Area of Science:

Cryobiology
Biotechnology
Materials Science

Background:

Vitrification and ultrarapid laser warming are essential for cryopreserving valuable cells like embryos and oocytes.
Current methods face challenges in precision and success rates for long-term storage.

Purpose of the Study:

To develop and evaluate a novel, integrated cryojig for enhanced precision in laser warming during cryopreservation.
To improve the accuracy and success rate of rewarming cryopreserved cells and tissues.

Main Methods:

Designed and fabricated a novel one-piece cryojig integrating jig tool and holder functionalities.
Utilized the cryojig in conjunction with vitrification and ultrarapid laser warming techniques.
Assessed laser accuracy and rewarming success rates post-cryopreservation.

Main Results:

The novel cryojig achieved a high laser accuracy of 95%.
A successful rewarming rate of 62% was recorded using the refined device.
The integrated cryojig demonstrated improved laser accuracy in warming after long-term vitrification storage.

Conclusions:

The developed cryojig significantly enhances laser accuracy and rewarming success in cryopreservation.
This innovation holds promise for advancing cryobanking applications for diverse species.
Further research can optimize this technique for broader cell and tissue preservation.