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Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
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UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.4K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.4K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
437
UV–Vis Spectrum01:30

UV–Vis Spectrum

1.2K
When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar...
1.2K
IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

4.3K
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
4.3K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

3.3K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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Related Experiment Video

Updated: Jul 29, 2025

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
15:25

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

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Non-Destructive Quality Evaluation of 80 Tomato Varieties Using Vis-NIR Spectroscopy.

Lilija Duckena1, Reinis Alksnis2, Ieva Erdberga1

  • 1Faculty of Agriculture, Institute of Soil and Plant Science, Latvia University of Life Sciences and Technologies, 2 Liela Street, LV-3001 Jelgava, Latvia.

Foods (Basel, Switzerland)
|May 27, 2023
PubMed
Summary

Visible and Near-Infrared (Vis-NIR) spectroscopy offers a rapid, non-destructive method for assessing tomato quality. This study demonstrates its effectiveness in predicting key attributes like lycopene and dry matter content.

Keywords:
Solanum lycopersicum L.Vis-NIR spectroscopypartial least squares regression (PLS)phytochemicals

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O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
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Area of Science:

  • Agricultural Science
  • Spectroscopy
  • Food Science

Background:

  • Traditional methods for determining fruit quality are time-consuming and resource-intensive.
  • Non-destructive spectral analysis offers a cost-effective alternative for fruit quality assessment.
  • Visible and Near-Infrared (Vis-NIR) spectroscopy is a widely adopted technique for this purpose.

Purpose of the Study:

  • To analyze internal quality attributes of intact tomatoes using Vis-NIR spectroscopy.
  • To develop predictive models for taste index, lycopene, flavonoids, β-carotene, total phenols, and dry matter.
  • To establish the capability of Vis-NIR spectroscopy for diverse tomato varieties.

Main Methods:

  • Utilized Vis-NIR reflectance spectra from 80 diverse tomato varieties.
  • Employed a portable spectroradiometer (RS-3500) for spectral data acquisition.
  • Developed calibration models using Partial Least Squares regression (PLS) and Multiple Scatter Correction (MSC).

Main Results:

  • Achieved high prediction accuracy for lycopene and dry matter content (R² = 0.90).
  • Successfully modeled taste index (R² = 0.86), flavonoids (R² = 0.84), β-carotene (R² = 0.82), and total phenols (R² = 0.73).
  • Demonstrated the efficacy of PLS models for predicting internal tomato quality.

Conclusions:

  • Vis-NIR spectroscopy is a highly capable tool for non-destructively determining internal quality attributes of intact tomatoes.
  • The developed models provide accurate predictions for key nutritional and sensory parameters.
  • This technique offers a valuable alternative to traditional biochemical analysis for quality control.