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Related Experiment Video

Updated: Jul 29, 2025

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Published on: September 21, 2021

Surgically Induced Demyelination in Rat Sciatic Nerve.

Rahul Shankar Rao Rayilla¹, Mur Naidu², Phanithi Prakash Babu¹

¹Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.

|May 27, 2023

Summary

A new surgical method induces demyelination in rat sciatic nerves. Teriflunomide treatment successfully promoted remyelination and restored motor function, offering a potential therapeutic avenue for peripheral nerve injuries.

Keywords:

demyelination remyelination sciatic nerve injury teriflunomide

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In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

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Last Updated: Jul 29, 2025

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines

Published on: September 21, 2021

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

Published on: March 13, 2012

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

Published on: February 10, 2023

Area of Science:

Neuroscience
Regenerative Medicine
Surgical Techniques

Background:

Peripheral nerve injuries (PNIs) often involve demyelination, damaging the myelin sheath around axons.
Current animal models for inducing peripheral nervous system (PNS) demyelination are limited.
Sciatic nerve damage is a common PNI model, but controlled demyelination induction is challenging.

Purpose of the Study:

To develop and validate a surgical technique for inducing demyelination in the rat sciatic nerve.
To investigate the effects of Teriflunomide (TF) on demyelination and functional recovery.
To establish a reliable preclinical model for studying PNI and potential treatments.

Main Methods:

A surgical partial sciatic nerve suture (p-SNI) was performed on young male Sprague Dawley rats.
Histology, immunostaining, and transmission electron microscopy (TEM) were used to assess demyelination, axonal damage, and myelin repair.
Motor function was evaluated using the rotarod test.
Teriflunomide (TF) was administered to assess its therapeutic effects.

Main Results:

The p-SNI model successfully induced varying degrees of demyelination and myelin loss.
Histological analysis revealed axonal atrophy and inter-axonal gaps post-injury.
Rotarod tests confirmed significant motor function deficits in p-SNI rats.
TF treatment led to motor function restoration, axonal repair, and evidence of remyelination.

Conclusions:

A novel surgical approach effectively induces demyelination in the rat sciatic nerve.
Teriflunomide demonstrates significant therapeutic potential in promoting remyelination and functional recovery after PNI.
This study provides a valuable model for investigating PNI and evaluating novel therapeutic interventions.