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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
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Monocyte Differentiation and Heterogeneity: Inter-Subset and Interindividual Differences.

Helen Williams1,2, Corinne Mack1,2, Rana Baraz1,2

  • 1Vascular Biology Research Centre, Department of Surgery, Westmead Hospital, Westmead, NSW 2145, Australia.

International Journal of Molecular Sciences
|May 27, 2023
PubMed
Summary

Human monocyte subsets (classical, intermediate, nonclassical) display complex heterogeneity. This variation exists within subsets, across health and disease states, and between individuals, impacting research and clinical relevance.

Keywords:
differentiationheterogeneityinflammationlipidmonocytemonocyte subsetstrained immunity

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Human monocytes comprise three main subsets: classical, intermediate, and nonclassical.
  • These subsets are distinguished by CD14 and CD16 expression and exhibit distinct phenotypes and functions.
  • Existing research acknowledges functional differences between these monocyte subsets.

Purpose of the Study:

  • To explore the multi-dimensional heterogeneity of human monocyte subsets.
  • To discuss the implications of intra-subset variation for monocyte classification and function.
  • To highlight the relevance of monocyte heterogeneity in health and disease.

Main Methods:

  • Phenotypic analysis of monocyte subsets using markers like CD14 and CD16.
  • Comparative studies of monocyte function in steady-state and disease conditions.
  • Investigation of interindividual variability in monocyte populations.

Main Results:

  • Monocyte heterogeneity is multi-dimensional, extending beyond distinct subset differences.
  • Significant heterogeneity is observed within each monocyte subset.
  • Interindividual differences in monocyte subsets exist even in healthy individuals, potentially influenced by microenvironmental factors.

Conclusions:

  • Understanding monocyte heterogeneity is crucial for accurate classification and functional assignment.
  • Intra-subset and interindividual variations significantly impact the study of monocytes in health and disease.
  • Microenvironmental influences may lead to long-lasting changes in monocyte precursors and their derived cells.