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Toxic Determination of Cry11 Mutated Proteins Obtained Using Rational Design and Its Computational Analysis.

Miguel O Suárez-Barrera1,2, Diego F Herrera-Pineda1, Paola Rondón-Villarreal1

  • 1Facultad de Ciencias Médicas y de la Salud, Instituto de Investigación Masira, Universidad de Santander, Bucaramanga 680003, Colombia.

International Journal of Molecular Sciences
|May 27, 2023
PubMed
Summary
This summary is machine-generated.

Mutations in Cry11 proteins significantly altered their toxicity against Aedes aegypti larvae. Specific amino acid changes reduced or eliminated insecticidal activity, offering insights for developing new pest control agents.

Keywords:
Aedes aegyptiBacillus thuringiensisCry11cancer cell linessite-directed mutagenesis

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Entomology

Background:

  • Cry11 proteins are insecticidal toxins produced by Bacillus thuringiensis.
  • Aedes aegypti mosquitoes transmit diseases like dengue, chikungunya, and Zika.
  • Previous research created a variant (variant 8) of Cry11Aa/Cry11Bb with deletions and substitutions.

Purpose of the Study:

  • To investigate the impact of specific mutations on Cry11 protein toxicity and stability.
  • To generate and characterize novel Cry11 variants for potential vector control applications.

Main Methods:

  • Site-directed mutagenesis was used to create specific amino acid substitutions in Cry11 variants.
  • Toxicity was assessed using median-lethal concentration (LC50) tests on Aedes aegypti larvae.
  • Cytotoxicity assays were performed on SW480 colorectal cancer cells.
  • Molecular dynamic simulations were employed to analyze protein structure and stability.

Main Results:

  • Mutants 8F553L, 8W556L, 8F553L/8W556L, and C157R lost significant toxic activity against Aedes aegypti.
  • The A92D mutant showed an 11.4-fold decrease in toxicity compared to Cry11Bb.
  • Variant 8 and its mutant 8W556L exhibited 30-50% cellular viability in SW480 cells.
  • Molecular simulations highlighted the role of mutations at positions 553 and 556 in Cry11 protein structure and activity.

Conclusions:

  • Specific mutations in Cry11 proteins critically affect their insecticidal activity against Aedes aegypti.
  • These findings provide valuable knowledge for designing improved Cry11-based biopesticides.
  • The study suggests potential for Cry11 proteins in vector control and cancer research.