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Updated: Jul 29, 2025

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
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Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

Published on: January 7, 2013

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Kidney-Related Function of Mitochondrial Protein Mitoregulin

Olga A Averina1,2, Oleg A Permyakov1, Mariia A Emelianova3

  • 1Institute of Functional Genomics, Lomonosov Moscow State University, 119992 Moscow, Russia.

International Journal of Molecular Sciences
|May 27, 2023
PubMed

Insights

Mitochondrial protein Mitoregulin (Mtln) deficiency impairs kidney function in aged mice, causing tubular degeneration in males and reduced filtration in females. This highlights Mtln

Area of Science:

  • Mitochondrial biology
  • Nephrology
  • Metabolic diseases

Background:

  • Mitochondria are crucial for kidney function, particularly oxidative phosphorylation.
  • Mitochondrial protein Mitoregulin (Mtln) is involved in energy metabolism.
  • Mtln knockout mice exhibit obesity and muscle mitochondrial dysfunction.

Purpose of the Study:

  • To investigate kidney-specific phenotypes in aged Mitoregulin (Mtln) knockout mice.
  • To assess the impact of Mtln deficiency on renal mitochondrial function and kidney health.

Main Methods:

  • Analysis of kidney mitochondrial respiratory complex I activity.
  • Assessment of cardiolipin damage in kidney mitochondria.
  • Histopathological examination of renal tubules and glomeruli.
  • Measurement of glomerular filtration rate (GFR).

Main Results:

  • Kidney mitochondria in Mtln knockout mice showed decreased respiratory complex I activity and increased cardiolipin damage.
  • Aged male Mtln knockout mice exhibited higher frequencies of renal proximal tubule degeneration.
  • Aged female Mtln knockout mice showed a higher incidence of decreased glomerular filtration rate.
  • The abundance of Mtln partner protein, Cyb5r3, was significantly reduced in the kidneys of knockout mice.

Conclusions:

  • Mitochondrial protein Mitoregulin (Mtln) deficiency leads to kidney dysfunction in aged mice.
  • Mtln is essential for maintaining kidney mitochondrial health and overall renal function.
  • Sex-specific kidney phenotypes are observed in the absence of Mtln, suggesting differential susceptibility.
Keywords:
kidneymetabolismmitochondriamitoregulinoxidative phosphorylationsmall peptide

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