Transcriptome Analysis Reveals Genes Associated with Flooding Tolerance in Mulberry Plants

Area of Science:

• Plant Biology
• Molecular Genetics
• Stress Physiology

Background:

• Mulberry (Morus alba) is an economically important plant known for its tolerance to flooding.
• The genetic basis of submergence tolerance in mulberry remains largely uncharacterized.

Purpose of the Study:

• To investigate the regulatory gene network responsible for submergence tolerance in mulberry.
• To identify key genes and pathways involved in the adaptation of mulberry to flooding stress.

Main Methods:

• Mulberry plants were subjected to submergence stress.
• Quantitative reverse-transcription PCR (qRT-PCR) and transcriptome analysis were performed on mulberry leaves.

Main Results:

• Genes related to reactive oxygen species (ROS) homeostasis, such as ascorbate peroxidase and glutathione S-transferase, were significantly upregulated.
• Upregulation of genes involved in glycolysis, ethanol fermentation, and the TCA cycle suggests mechanisms to mitigate energy deficits.
• Genes associated with hormone signaling (ethylene, cytokinin, abscisic acid, MAPK), phenylpropanoid biosynthesis, and transcription factors were also upregulated.

Conclusions:

• The findings elucidate the complex gene regulatory network underlying submergence tolerance in mulberry.
• Identified genes provide insights into mulberry's adaptation strategies to flooding and potential targets for molecular breeding.