Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Controls-I

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Asymmetric calcification of the extracardiac Fontan conduit: Quantitative insights from long-term computed tomography imaging.

Annals of pediatric cardiology·2026

Same author

From Phytochemistry to Drug Discovery: A Systematic Review of Multitarget Pharmacology and Lead Compound Prioritization of <i>Piper crocatum</i> Ruiz & Pav.

Drug design, development and therapy·2026

Same author

Association between infrapatellar fat pad stiffness and gait-related mechanical characteristics in women with knee osteoarthritis.

Clinical biomechanics (Bristol, Avon)·2026

Same author

Pre-weekend effect in pulmonary resection for non-small cell lung cancer: a multicenter retrospective cohort study.

Surgery today·2026

Same author

Pulse Driven Injection in an Additive-Manufactured Microchannel for Rapid Mixing of Stratified Concurrent Flow and On-Demand Droplet Generation.

Micromachines·2026

Same author

REgistry Of Catheter AbLation After Congenital Heart Disease Surgery(REAL-CHD Registry).

Journal of arrhythmia·2026

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

High-Efficiency Single-Cell Containment Microdevices Based on Fluid Control.

Daiki Tanaka¹, Junichi Ishihara², Hiroki Takahashi^2,3,4

¹Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda Tsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.

|May 27, 2023

Summary

Researchers developed a novel comb-shaped microfluidic device for efficient single bacterium trapping and culturing. This new device significantly improves storage efficiency and allows rapid chemical exchange, benefiting studies on resistant bacteria.

Keywords:

cell culture fluid control microfluidic devices single cell

More Related Videos

A Microfluidic-based Hydrodynamic Trap for Single Particles

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

A Microfluidic-based Hydrodynamic Trap for Single Particles

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Area of Science:

Microfluidics
Cell Biology
Bacteriology

Background:

Conventional methods struggle with single bacterium trapping and require centrifugation.
Existing devices present challenges in efficient cell storage and rapid chemical manipulation.
Culturing resistant bacteria necessitates advanced microfluidic solutions.

Purpose of the Study:

To develop an efficient microfluidic device for single bacterium trapping and culturing.
To enhance storage efficiency and enable rapid chemical exchange for bacterial studies.
To investigate pressure dynamics within microfluidic growth channels.

Main Methods:

Fabrication of a comb-shaped microfluidic device using soft microelectromechanical systems (MEMS).
Utilizing flowing fluid for bacteria storage within growth channels.
Employing computational simulations to analyze pressure loss in growth channels.

Main Results:

Achieved a significant improvement in storage efficiency from 0.2% to 84% using microbeads.
Demonstrated reduced pressure loss in the growth channel (<400 PaG) compared to conventional devices (>1400 PaG).
Successfully applied the device to culture diverse bacteria, including *Salmonella enterica* and *Staphylococcus aureus*.

Conclusions:

The developed microfluidic device offers superior performance for single-cell trapping and culturing.
Its rapid chemical exchange capability is ideal for studying resistant bacterial strains.
The device's versatility and ease of fabrication make it broadly applicable in microbiology research.