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Related Experiment Video

Updated: Jul 29, 2025

Expression and Purification of Virus-like Particles for Vaccination
06:17

Expression and Purification of Virus-like Particles for Vaccination

Published on: June 2, 2016

22.0K

One Step Purification-Vaccine Delivery System.

Ernesto R Soto1, Charles A Specht2, Chrono K Lee2

  • 1Program in Molecular Medicine, UMass Chan Medical School, Worcester, MA 01605, USA.

Pharmaceutics
|May 27, 2023
PubMed
Summary
This summary is machine-generated.

Glucan particles (GPs) were modified with nickel nanoparticles (GP-Ni) for efficient binding of histidine-tagged proteins. This novel vaccine delivery system targets antigen-presenting cells (APCs) effectively, showing comparable results to previous methods.

Keywords:
glucan particleshistidine-tagged proteinsnickel nanoparticlesvaccine

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Area of Science:

  • Biotechnology
  • Immunology
  • Nanotechnology

Background:

  • Glucan particles (GPs) are yeast-derived microspheres with a 1,3-β-glucan shell.
  • Their structure facilitates receptor-mediated uptake by innate immune cells like macrophages.
  • GPs are established carriers for targeted payload delivery, including vaccines and nanoparticles.

Purpose of the Study:

  • To develop a novel method for preparing GP-encapsulated nickel nanoparticles (GP-Ni).
  • To enable one-step binding of histidine (His)-tagged proteins for vaccine applications.
  • To evaluate the efficacy of GP-Ni-encapsulated antigens in a preclinical model.

Main Methods:

  • Preparation of GP-encapsulated nickel nanoparticles (GP-Ni).
  • Encapsulation of His-tagged Cda2 cryptococcal antigens within GP-Ni.
  • Assessment of the GP-Ni-Cda2 vaccine in a mouse infection model.

Main Results:

  • The GP-Ni system effectively binds His-tagged proteins, such as Cda2 antigens.
  • The GP-Ni-Cda2 vaccine demonstrated comparable efficacy to previous GP-based vaccine strategies.
  • This method facilitates targeted delivery to antigen-presenting cells (APCs).

Conclusions:

  • The novel GP-Ni approach offers a streamlined method for vaccine antigen encapsulation.
  • This technology enhances targeted vaccine delivery to APCs for improved immune responses.
  • The GP-Ni system holds promise for antigen discovery and advanced vaccine development.