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Updated: Aug 12, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
Sonal Fande1, Sangam Srikanth2, Jayapiriya U S1
1Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronic (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India.
Abstract:
Here, we present a protocol for a miniaturized microfluidic device that enables quantitative tracking of bacterial growth. We describe steps for fabricating a screen-printed electrode, a laser-induced graphene heater, and a microfluidic device with its integrations. We then detail the electrochemical detection of bacteria using a microfluidic fuel cell. The laser-induced graphene heater provides the temperature for the bacterial culture, and metabolic activity is recognized using a bacterial fuel cell. Please see Srikanth et al.1 for comprehensive information on the application and execution of this protocol.
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