Lethal and sublethal implications of low temperature exposure for three intertidal predators

Benthic invertebrate predators play a key role in top-down trophic regulation in intertidal ecosystems. While the physiological and ecological consequences of predator exposure to high temperatures during summer low tides are increasingly well-studied, the effects of cold exposure during winter low tides remain poorly understood. To address this knowledge gap, we measured the supercooling points, survival, and feeding rates of three intertidal predator species in British Columbia, Canada - the sea stars Pisaster ochraceus and Evasterias troschelii and the dogwhelk Nucella lamellosa - in response to exposure to sub-zero air temperatures. Overall, we found that all three predators exhibited evidence of internal freezing at relatively mild sub-zero temperatures, with sea stars exhibiting an average supercooling point of -2.50 °C, and the dogwhelk averaging approximately -3.99 °C. None of the tested species are strongly freeze tolerant, as evidenced by moderate-to-low survival rates after exposure to -8 °C air. All three predators exhibited significantly reduced feeding rates over a two-week period following a single 3-h sublethal (-0.5 °C) exposure event. We also quantified variation in predator body temperature among thermal microhabitats during winter low tides. Predators that were found at the base of large boulders, on the sediment, and within crevices had higher body temperatures during winter low tides, as compared to those situated in other microhabitats. However, we did not find evidence of behavioural thermoregulation via selective microhabitat use during cold weather. Since these intertidal predators are less freeze tolerant than their preferred prey, winter low temperature exposures can have important implications for organism survival and predator-prey dynamics across thermal gradients at both local (habitat-driven) and geographic (climate-driven) scales.