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Updated: Jul 29, 2025

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
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Loss of Residues 119-136, Including the First β-strand of Human Prion Protein, Generates an Aggregation-competent

Laszlo L P Hosszu1, Daljit Sangar1, Mark Batchelor1

  • 1MRC Prion Unit at UCL, UCL Institute of Prion Diseases, 33 Cleveland Street, London W1W 7FF, UK(†).

Journal of Molecular Biology
|May 27, 2023
PubMed
Summary

Prion protein (PrPC) structural conversion is key to prion disease. Removing specific residues (119-136) creates an "open" PrP conformer that fibrillizes more readily, suggesting a stepwise folding transition.

Keywords:
biophysicshydrogen-deuterium exchangeneurodegenerationprion protein

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