Metabolomics as a tool to elucidate biochemical cold adaptation in insects

Metabolomics is an incredibly valuable tool in helping understand insect responses to cold. It not only characterizes how low temperature disrupts metabolic homeostasis, but also how it triggers fundamental adaptive responses, for example, homeoviscous adaptation and cryoprotectant accumulation. This review outlines the advantages and disadvantages of different metabolomic technologies (nuclear magnetic resonance- versus mass spectrometry-based) and screening approaches (targeted versus untargeted). We emphasize the importance of time-series and tissue-specific data, as well as the challenges of disentangling insect versus microbiome responses. In addition, we set out the need to move beyond simple correlations between metabolite abundance and tolerance phenotypes by undertaking functional assessments, for example, using dietary supplementation or injections. We highlight studies at the vanguard of employing these approaches, and where key knowledge gaps remain.