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Assessment of filter subsampling and extrapolation for quantifying microplastics in environmental samples using Raman
Dounia El Khatib1, Troy D Langknecht1, Michaela A Cashman2
1Oak Ridge Institute of Science Education, c/o U.S. Environmental Protection Agency, ORD/CEMM Atlantic Coastal Environmental Sciences Division, Narragansett, RI, USA.
Marine Pollution Bulletin
|May 28, 2023
Summary
This study introduces an efficient subsampling method for analyzing microplastics (MPs) on filters using Raman spectroscopy. A 12.5% filter subsample accurately estimates total MP contamination in sediments.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Materials Science
Background:
- Characterizing microplastics (MPs) typically involves filter capture and Raman spectroscopy.
- Manual scanning of entire filters is time-consuming and labor-intensive.
- Efficient quantification methods are crucial for assessing MP pollution.
Purpose of the Study:
- To develop and validate a subsampling method for Raman spectroscopic analysis of MPs (45-1000 µm) in sediments.
- To optimize the subsampling fraction for accurate MP quantification.
- To apply the method for assessing marine sediment contamination.
Main Methods:
- Developed a subsampling technique for MP analysis on filters.
- Evaluated the method using spiked MPs in water and sediment samples.
- Utilized statistical analysis to determine optimal subsample size and shape.
- Applied an extrapolation method for quantification.
Main Results:
- A subsampling fraction of 12.5% of the filter, in a wedge shape, was found to be optimal.
- This subsampling method proved efficient and accurate for estimating total MP counts.
- The method was successfully applied to assess MP contamination in US marine sediments.
Conclusions:
- Subsampling significantly reduces the time and labor required for MP analysis via Raman spectroscopy.
- The 12.5% wedge subsampling method provides a reliable and accurate approach for MP quantification.
- This optimized method facilitates broader assessment of microplastic contamination in marine environments.
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