Malathion detection based on polydopamine enhanced oxidase-mimetic activity of palladium nanocubes

Nowadays, fabricating simple and efficient pesticide detection methods become a research focus due to the great threat pesticide residues posed to human health and environment. Herein, we constructed a high-efficiency and sensitive colorimetric detection platform for malathion detection based on polydopamine-dressed Pd nanocubes (PDA-Pd/NCs). The Pd/NCs coated with PDA exhibited excellent oxidase-like activity, which was attributed to the substrates accumulation and accelerated electron transfer induced by PDA. What's more, we successfully achieved sensitive detection of acid phosphatase (ACP) using 3,3',5,5'-tetramethylbenzidine (TMB) as the chromogenic substrate, relying on the satisfactory oxidase activity from PDA-Pd/NCs. However, the addition of malathion could inhibit the activity of ACP and limit the production of medium AA. Therefore, we constructed a colorimetric assay for malathion based on PDA-Pd/NCs + TMB + ACP system. The wide linear range (0-8 μM) and low detection limit (0.023 μM) indicate excellent analytical performance, which is superior to most malathion analysis methods previously reported. This work not only provides a new idea for dopamine coated nano-enzyme to improve its catalytic activity, but also creates a new tactics for the detection of pesticides such as malathion.