Fluoride resistance capacity in mammalian cells involves global gene expression changes associate with ferroptosis

Area of Science:

• Cell Biology
• Toxicology
• Genomics

Background:

• High fluoride environments pose risks to bone health.
• Understanding cellular responses to fluoride is crucial for treating fluorosis.

Purpose of the Study:

• Investigate mouse osteoblast ferroptosis under high fluoride conditions.
• Identify genetic mechanisms of fluoride resistance.
• Provide a basis for fluorosis treatment.

Main Methods:

• Mouse osteoblast cell line MC3T3-E1 exposed to varying fluoride concentrations.
• Assays for cell viability, reactive oxygen species, and lipid peroxidation.
• High-throughput RNA sequencing to identify differentially expressed genes.

Main Results:

• Elevated fluoride levels (20-90 ppm) decreased cell viability and increased oxidative stress and lipid peroxidation.
• High-throughput sequencing revealed 2702 differentially expressed genes in fluoride-resistant cells.
• 17 of these genes were identified as ferroptosis-related.

Conclusions:

• High fluoride exposure induces ferroptosis in osteoblasts.
• Ferroptosis-related genes play a significant role in cellular fluoride resistance.
• Findings contribute to understanding fluorosis pathogenesis and treatment strategies.