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Biological differences between FIM2 and FIM3 fimbriae of Bordetella pertussis: not just the serotype

Soraya Matczak1, Valérie Bouchez2, Pauline Leroux1

  • 1Institut Pasteur, Université Paris Cité, Biodiversity and Epidemiology of Bacterial Pathogens, 28, Rue Du Docteur Roux, 75015, Paris, France.

Microbes and Infection
|May 28, 2023
PubMed
Abstract

Insights

Bordetella pertussis serotypes FIM2 and FIM3 show distinct proteomic and biological differences. These variations in fimbrial serotypes and fim3 clades impact pathogenesis and the emergence of pertussis.

Area of Science:

  • Microbiology
  • Immunology
  • Genomics

Background:

  • Bordetella pertussis circulates globally despite vaccination efforts.
  • Fimbriae are key components in acellular pertussis vaccines.
  • Distinct population dynamics exist for B. pertussis fimbrial serotypes (FIM2, FIM3) and fim3 alleles (clade 1, clade 2).

Purpose of the Study:

  • To compare microbiological characteristics and protein expression between FIM2 and FIM3 serotypes.
  • To analyze differences in relation to genomic clades of B. pertussis.

Main Methods:

  • Analysis of 19 B. pertussis isolates.
  • Assessment of virulence factors, autoagglutination, biofilm formation, and bacterial survival in whole blood.
  • Evaluation of induced cytokine secretion and global proteome profiling.

Main Results:

  • FIM2 isolates exhibited higher fimbriae and biofilm production, but less pertussis toxin and autoagglutination compared to FIM3.
  • FIM2 isolates showed reduced survival in cord blood but induced higher IL-4, IL-8, and IL-1β levels.
  • Proteomic analysis revealed 15 differentially expressed proteins related to adhesion and metal metabolism; FIM3 clade 2 isolates produced more FIM3 and biofilm than clade 1.

Conclusions:

  • Fimbrial serotype and fim3 clades are linked to significant proteomic and biological variations in B. pertussis.
  • These differences may influence the pathogenesis and epidemiological spread of pertussis.
Keywords:
Bordetella pertussisFimbriaePhylogenetic cladeProteomicsSerotypeVirulence

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