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Updated: Jul 28, 2025
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Published on: June 30, 2018
Spontaneously Blinking Rhodamine Dyes for Single-Molecule Localization Microscopy
Weijie Chi1,2, Davin Tan2, Qinglong Qiao3
1Collaborative Innovation Center of One Health, School of Science, Hainan University, Renmin Road 58, Haikou, 570228, P. R. China.
Abstract:
Single-molecule localization microscopy (SMLM) has found extensive applications in various fields of biology and chemistry. As a vital component of SMLM, fluorophores play an essential role in obtaining super-resolution fluorescence images. Recent research on spontaneously blinking fluorophores has greatly simplified the experimental setups and extended the imaging duration of SMLM. To support this crucial development, this review provides a comprehensive overview of the development of spontaneously blinking rhodamines from 2014 to 2023, as well as the key mechanistic aspects of intramolecular spirocyclization reactions. We hope that by offering insightful design guidelines, this review will contribute to accelerating the advancement of super-resolution imaging technologies.
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