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Dispersive solid-phase extraction of alkaloids and polyphenols using borate hypercrosslinked polymers.

Xiaobing Liu1, Pan Wang1, Kai Hu1

  • 1Henan University of Chinese Medicine, Zhengzhou, P. R. China.

Journal of Separation Science
|May 28, 2023
PubMed
Summary

A novel borate polymer effectively adsorbs alkaloids and polyphenols. This enables sensitive, simultaneous quantification in herbal products using ultra-high performance liquid chromatography.

Keywords:
alkaloidsdispersive solid-phase extractionhigh-performance liquid chromatographyhyper-crosslinked polymerspolyphenols

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Polymer Chemistry

Background:

  • Alkaloids and polyphenols are important bioactive compounds found in various herbal products.
  • Accurate quantification of these compounds is crucial for quality control and pharmacological studies.
  • Existing methods may lack sensitivity or require complex sample preparation.

Purpose of the Study:

  • To synthesize a novel borate hyper-crosslinked polymer for efficient adsorption of alkaloids and polyphenols.
  • To develop a sensitive analytical method for simultaneous determination of these compounds in complex matrices.
  • To evaluate the adsorption performance and analytical characteristics of the developed material and method.

Main Methods:

  • Synthesis of a borate hyper-crosslinked polymer via Friedel-Crafts reaction.
  • Characterization of adsorption performance (capacity, kinetics, isotherms) for alkaloids and polyphenols.
  • Development and validation of an ultra-high performance liquid chromatography (UHPLC) method coupled with the synthesized polymer for sample extraction and analysis.

Main Results:

  • The synthesized polymer demonstrated excellent adsorption capacities for alkaloids and polyphenols (25.07–39.60 mg/g).
  • Adsorption followed a monolayer and chemical process, as indicated by kinetic and isotherm models.
  • The developed UHPLC method showed a wide linear range (5.0–5000.0 ng/ml), low limits of detection (0.66–11.25 ng/ml), and good recoveries (81.2%–117.4%) in green tea and Coptis chinensis.

Conclusions:

  • A borate hyper-crosslinked polymer was successfully synthesized with high adsorption capabilities for alkaloids and polyphenols.
  • A sensitive and reliable UHPLC method was established for the simultaneous determination of these compounds in herbal products.
  • The developed method offers a simple and convenient approach for analyzing alkaloids and polyphenols in complex natural samples.