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Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

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Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?

Hasan Alkan¹, Fatma Satilmis¹, Mehmet Akif Demirel¹

¹Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.

Reproduction in Domestic Animals = Zuchthygiene

|May 29, 2023

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The microfluidic sperm sorting chip improves cattle embryo development and quality in in vitro embryo production (IVEP). This method increases blastocyst rates and reduces apoptosis compared to routine sperm treatment.

Area of Science:

Reproductive Biology
Animal Science
Biotechnology

Background:

In vitro embryo production (IVEP) in cattle is crucial for genetic improvement.
Sperm quality significantly impacts IVEP success rates.
Current sperm preparation methods may not optimize sperm selection for fertilization.

Purpose of the Study:

To evaluate the efficacy of a microfluidic sperm sorting chip (MFSC) in improving embryo development and quality during bovine IVEP.
To compare the outcomes of using MFSC-prepared sperm versus routine sperm treatment.

Main Methods:

Holstein cattle oocytes were matured in vitro and randomly assigned to two groups.
The experimental group utilized sperm processed with a microfluidic sperm sorting chip (MFSC).

Keywords:

IVEP apoptosis cleavage rate rate of reaching blastocyst sperm treatment

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The control group used sperm prepared via standard commercial company protocols.

Main Results:

The MFSC group showed significantly higher cleavage rates (85.71% vs. 76.33%) and blastocyst development rates (44.15% vs. 32.54%).
Embryos from the MFSC group exhibited increased cell counts for Inner Cell Mass (ICM), Trophectoderm (TE), and Total Cells (TC).
Apoptosis rates were significantly lower in blastocysts derived from MFSC-treated sperm (3.06% vs. 7.72%).

Conclusions:

Microfluidic sperm sorting chip technology enhances embryo development, quality, and blastocyst formation rates in bovine IVEP.
MFSC reduces apoptosis in developing bovine embryos, suggesting improved gamete quality.
This technology presents a promising alternative for sperm preparation in cattle IVEP.