Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response...

Area of Science:

• Pharmacology
• Traditional Chinese Medicine
• Herbal Medicine Processing

Background:

• Aconitum pendulum Busch. (Tiebangchui, TBC) is a potent Tibetan medicine with significant toxicity, posing risks due to narrow therapeutic and toxic dose ranges.
• Existing Tibetan medicine literature suggests stir-frying TBC with Zanba as a processing method to mitigate toxicity, but specific parameters are undefined.
• There is an urgent need for a safe and effective method to reduce TBC's toxicity for clinical application and industrial production.

Purpose of the Study:

• To optimize and standardize the processing technology of Zanba-stir-fried TBC.
• To establish safe and effective processing parameters for Zanba-stir-fried TBC.
• To provide an experimental basis for the safe clinical use and industrial production of TBC.

Main Methods:

• A single-factor experiment was conducted to evaluate TBC slice thickness, Zanba quantity, processing temperature, and time.
• CRITIC method combined with the Box-Behnken response surface methodology was employed for optimization.
• Monoester and diester alkaloid content in Zanba-stir-fried TBC served as the primary indexes for evaluating toxicity reduction.

Main Results:

• The optimized processing conditions for Zanba-stir-fried TBC were determined as: TBC slice thickness of 2 cm, Zanba quantity three times that of TBC, processing temperature of 125 °C, and a stir-frying time of 60 minutes.
• These optimized parameters are expected to significantly reduce the toxicity of TBC while preserving its therapeutic efficacy.
• The study successfully established standardized processing conditions for Zanba-stir-fried TBC.

Conclusions:

• The optimized Zanba-stir-frying method provides a safe and effective approach to reduce the inherent toxicity of Aconitum pendulum Busch. (TBC).
• This standardized processing technology is crucial for the safe clinical application and large-scale industrial production of TBC.
• The findings lay the groundwork for further research into the pharmacological activities and safety profiles of processed TBC.