Lignocellulosic-Based Nanoparticles with Photoluminescent Properties for Bioimaging

Area of Science:

• Biomaterials Science
• Nanotechnology
• Bioimaging

Background:

• Natural resources like lignin offer biocompatible and abundant biomaterials.
• Lignin, a plant biopolymer, forms lignocellulosic materials with diverse applications.
• Biomaterials derived from waste sources are increasingly important for sustainability.

Purpose of the Study:

• To develop and characterize novel lignocellulosic-based nanoparticles for bioimaging.
• To evaluate the photoluminescent properties and biocompatibility of these nanoparticles.
• To explore the potential of plant-derived waste as a source for advanced bioimaging agents.

Main Methods:

• Preparation of lignocellulosic-based nanoparticles from rose biomass waste.
• Characterization of nanoparticle size (average 156 nm).
• Assessment of photoluminescence (excitation 500 nm, emission 800 nm NIR).
• Evaluation of in vitro cytotoxicity (IC50 ~3 mg/mL) and in vivo toxicity (up to 57 mg/kg).
• Observation of blood circulation and urinary excretion in vivo.

Main Results:

• Lignocellulosic nanoparticles exhibit strong photoluminescence in the near-infrared (NIR) region.
• The nanoparticles possess inherent luminescent properties, negating the need for external agents.
• Demonstrated low in vitro and in vivo toxicity, with no adverse effects observed at tested doses.
• Nanoparticles showed sustained blood circulation and efficient excretion via urine.
• The average particle size was determined to be 156 nm.

Conclusions:

• Lignin-based nanoparticles derived from biomass waste are effective bioimaging agents.
• Their strong NIR luminescence, biocompatibility, and low toxicity support their use in biological applications.
• These nanoparticles represent a sustainable and efficient alternative to conventional bioimaging agents.