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Characterization of a novel phage vB_Pae_HB2107-3I that infects Pseudomonas aeruginosa

Jiuna Kong1, Guanhua Xuan1, Hong Lin1

  • 1Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.

Molecular Genetics and Genomics : MGG
|May 29, 2023
PubMed

Insights

Bacteriophages, or phages, are promising alternatives to antibiotics for treating drug-resistant bacteria. This study details a novel phage, vB_Pae_HB2107-3I, effective against multi-drug resistant Pseudomonas aeruginosa.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Antibiotic resistance in bacteria, particularly Pseudomonas aeruginosa, poses a significant global health threat.
  • Bacteriophages (phages) are viruses that infect bacteria and are being explored as alternatives to conventional antibiotics.
  • Developing novel phages with broad host ranges and stability is crucial for phage therapy.

Purpose of the Study:

  • To sequence and characterize the genome of a novel double-stranded DNA podovirus, vB_Pae_HB2107-3I.
  • To evaluate the stability and lytic activity of vB_Pae_HB2107-3I against multi-drug resistant Pseudomonas aeruginosa.
  • To determine the phylogenetic relationship of vB_Pae_HB2107-3I within the Caudovirales order.

Main Methods:

  • Whole-genome sequencing of bacteriophage vB_Pae_HB2107-3I.
  • Stability assays across a range of temperatures (37-60 °C) and pH values (pH 4-12).
  • One-step growth curve analysis to determine latent period and burst size.
  • Bioinformatic analysis including open reading frame (ORF) prediction, functional annotation, and phylogenetic analysis.

Main Results:

  • The genome of vB_Pae_HB2107-3I is 45,929 bp with 57% G+C content, encoding 72 ORFs (22 with predicted functions).
  • Phage vB_Pae_HB2107-3I demonstrated stability across tested temperatures and pH ranges, with a latent period of 10 min and a final titer of 8.1 × 10^9 PFU/mL at MOI 0.01.
  • Genome analysis confirmed the lysogenic nature of the phage, and phylogenetic analysis identified it as a novel Caudovirales member infecting P. aeruginosa.

Conclusions:

  • Phage vB_Pae_HB2107-3I is a stable, novel phage with potential as a biocontrol agent against Pseudomonas aeruginosa infections.
  • The characterization of this phage expands the knowledge of Pseudomonas phages and supports their development for therapeutic applications.
  • This research contributes to the growing body of evidence supporting bacteriophage therapy as a viable alternative to antibiotics for resistant bacterial infections.
Keywords:
Genome analysisMorphologyPhage vB_Pae_HB2107-3IPseudomonas aeruginosaStability

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