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Updated: Jul 28, 2025

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
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The Expression and Role of NADPH Oxidase 2 in Colon Cancer

Koichi Takiguchi1, Hiroki Shimizu2, Katsutoshi Shoda1

  • 1First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Anticancer Research
|May 29, 2023
PubMed
Abstract

Insights

Nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) is expressed in colon cancer and linked to poorer patient prognosis. Inhibiting NOX2 reduced cancer cell proliferation and motility, suggesting its potential as a biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Nicotinamide adenine dinucleotide phosphate oxidases (NOXs) are implicated in cancer progression.
  • The role of NOX2 in colon cancer remains underexplored.

Purpose of the Study:

  • To investigate the expression and pathophysiological roles of NOX2 in colon cancer.
  • To evaluate NOX2 as a prognostic biomarker in colon cancer patients.

Main Methods:

  • Immunohistochemistry of NOX2 in 116 colon cancer samples.
  • Correlation analysis of NOX2 expression with clinicopathological factors and survival.
  • NOX2 siRNA transfection in HCT116 and RKO colon cancer cell lines to assess functional impact.

Main Results:

  • NOX2 protein expression was observed in 38.8% of colon cancer tissues.
  • NOX2 expression was not significantly associated with clinicopathological factors.
  • Patients with NOX2-positive tumors had a significantly lower 5-year recurrence-free survival rate.
  • NOX2 depletion inhibited colon cancer cell proliferation and motility.

Conclusions:

  • NOX2 expression is associated with poor prognosis in colon cancer.
  • NOX2 influences colon cancer cell proliferation and motility.
  • NOX2 may serve as a valuable prognostic biomarker for colon cancer.
Keywords:
NADPH oxidase 2biomarkercolon cancerimmunohistochemistrytransfection

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