Attenuative Effect of Diallyl Trisulfide on Caspase Activity in TNF-α-induced Triple Negative Breast Cancer Cells
Konan J W Kanga1, Lambert H B Kanga2, Patricia Mendonca3
1Division of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Institute of Public Health, Florida A&M University, Tallahassee, FL, U.S.A.
Background/Aim:
Diallyl trisulfide (DATS) has been shown to prevent and inhibit carcinogenesis in cancer cells. We have previously shown DATS's ability to decrease the percentage of viable cells, inhibit cell migration and modulate genes involved in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) and mitogen-activated protein kinase (MAPK) signaling.
Materials And Methods:
This study aimed to compare the efficacy of DATS in tumor necrosis factor alpha (TNF-α) induced MDA-MB-231 and MDA-MB-468 cells and investigate its role in cell-death signaling via cell cycle, flow cytometry, and caspase assay.
Results:
DATS exhibit a time-dependent accumulation of G2/M phase cells in both cell lines, with higher effects in the MDA-MB-468 for all time points. DATS's ability to decrease the percentage of viable cells in both MDA-MB-231 and MDA-MB-468 cells was shown by a significant but slight increase of early and late apoptosis in the presence of DATS compared to control. Moreover, MDA-MB-468 cells showed more sensitivity to the DATS effect, evidenced by the higher percentage of apoptosis than MDA-MB-231 cells. The caspase studies showed a significant increase in caspase 3 and 8 activity in the presence of DATS, compared to control, in both cell lines. DATS showed no significant increase in caspase 9 activity in both cell lines compared to the control.
Conclusion:
DATS-induced apoptosis in human breast cancer cells is mediated, at least in part, by cell cycle arrest and caspase activity. These findings provide information for future studies into the role of DATS in TNBC therapy and prevention.
Insights
Diallyl trisulfide (DATS) induces apoptosis in human breast cancer cells by arresting the cell cycle and activating caspases. This study highlights DATS
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Diallyl trisulfide (DATS) demonstrates potential in preventing and inhibiting carcinogenesis.
- Previous research shows DATS decreases viable cancer cells, inhibits migration, and modulates NF-κB and MAPK signaling pathways.
Purpose of the Study:
- To compare the efficacy of DATS in tumor necrosis factor alpha (TNF-α) induced MDA-MB-231 and MDA-MB-468 breast cancer cells.
- To investigate DATS's role in cell-death signaling, focusing on cell cycle progression and caspase activation.
Main Methods:
- Cell cycle analysis using flow cytometry.
- Apoptosis assays to quantify cell death.
- Caspase activity assays (caspase 3, 8, and 9).
Main Results:
- DATS induced a time-dependent G2/M phase cell cycle arrest in both cell lines, more pronounced in MDA-MB-468 cells.
- DATS significantly increased early and late apoptosis in both cell lines, with greater sensitivity observed in MDA-MB-468 cells.
- Significant increases in caspase 3 and 8 activity were observed with DATS treatment, while caspase 9 activity remained unchanged.
Conclusions:
- DATS-induced apoptosis in human breast cancer cells is mediated by cell cycle arrest and caspase activation.
- Findings suggest DATS's therapeutic potential in triple-negative breast cancer (TNBC) treatment and prevention.
More Related Videos
08:49Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
Published on: January 22, 2019
13:38Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
Published on: January 18, 2017
Related Concept Videos
01:24Caspases
01:17The Extrinsic Apoptotic Pathway
01:31The Intrinsic Apoptotic Pathway